Single Cell Sequencing
Single cell RNA seq: Explore and interpret single cell gene expression profiles
Offering both microfluidics-based and combinatorial-barcoding single cell methods
Transcriptomics of individual cells can allow researchers to gain insights into cellular heterogeneity, identify rare cell types, and uncover new biomarkers for diseases.
SAGC has a Chromium Controller and Chromium X Series from 10X Genomics to enable multiomic analysis through droplet GEM-based single cell partitioning and sequencing.
Applications available:
- Single cell RNA expression (GEX)
- Single cell Immune Profiling (5' with TCR/BCR options)
- Single cell ATAC
- Single cell ATAC + GEX
- Single cell CNV
- Cell surface marker tagging can be incorporated into some of these techniques and quantified during sequencing.
Chromium GEM-X is NOW AVAILABLE
Updates to the reagent chemistry and microfluidics for both the 3’ Single Cell Gene Expression and the 5’ Immune Profiling solutions.
Chromium single cell gene expression (Flex)
- Single Cell Gene Expression from Fixed Cells (FFPE compatible)
High throughput and workflow efficiency:
run 1 to 128 samples, or up to 1 million cells per run. - Compatible with fixed, fresh, and frozen samples, as well as whole cells and nuclei.
Other single cell technologies (such as EvercodeTM from Parse Biosciences) leverage serial plating as an alternative to 10X, which relies on microfluidics. This can offer some advantages with certain cell types and achieve higher throughput at a lower cost.
Parse Biosciences: leverages combinatorial barcoding
- Scalable: 10K to 1M cells or nuclei
- Flexible: Fix and store samples collected on different days for the same run
- Time course study
- Collection of cells across different sites
- Very high-throughput experiments
- Sensitive detection of very rare cell types
Single cell experiments 'at scale' - the million cell experiment
