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Sample Requirements

Samples

All samples submitted for genomics services are required to meet strict quantity and quality thresholds. Samples outside of these thresholds may be able to be submitted (only by prior agreement by the centre), however, no guarantees will be given on the service outcomes for these. In general, 2 aliquots of samples are required at submission: one for initial QC and one for the service analysis.

Quantity

In general, a fluorescence assay using an appropriate dye (eg by Qubit assay) is the preferred way to measure the RNA or DNA concentration in a sample. Nanodrop (UV/Vis) measurements are prone to overestimation when contaminants are present and should only be used where sample concentrations are higher (>100ng/uL).

Quality

Total RNA and DNA (gDNA, amplicon etc) is assessed by a microfluidic assay (eg Agilent tapestation or PE Labchip) to ensure acceptable for the particular assay. While typical absorbance measurements (eg 260:280) are a good indication of purity they do not indicate whether a sample is degraded (integrity).

Data

NGS data is released once final sequencing specifications have been met. Sequencing services which do not meet the minimum manufacturer quality specifications will be rerun gratis (except where clients have agreed to run samples, or prepared pools, which do not meet the quality guidelines, and have agreed to proceed regardless).